Teloseq 12-plex Human and Cow cell lines

The Oxford Nanopore Technologies multiplex Telo-seq protocol can be used to sequence native telomeres and, coupled with the EPI2ME analysis workflow wf-teloseq, provides both global and chromosome-arm specific telomere length estimation. The method allows multiplexing of up to 12 samples on a single flow cell. This post describes a new Telo-seq 12-plex dataset release covering data for two human cell lines (HG002, 6 samples; HG005, 3 samples), and cow peripheral blood mononuclear cells (PBMCs, 3 samples), multiplexed together and sequenced on a PromethION and a GridION Flow Cell.

Dataset

The dataset is available for anonymous download, without login, from a public Amazon Web Services S3 bucket. The bucket is part of the Open Data on AWS project enabling sharing and analysis of a wide range of data. The data can be downloaded with the AWS CLI command:

aws s3 sync --no-sign-request s3://ont-open-data/wf_teloseq_LC2025 wf_teloseq_LC2025

See the tutorials page for information on downloading the dataset. You can also browse and download the files in your web browser courtesy of 42basepairs.

Sample

Preparation

Sample preparation was performed according to protocols published on the main Oxford Nanopore Technologies’ website.

Further preparation information such as sample storage suggestions can be found at https://nanoporetech.com/documentation/prepare.

Sequencing

Sequence data was generated using the following configuration:

Analysis

Analysis outputs are available. The analysis results are located in the S3 bucket under the prefix:

s3://ont-open-data/wf_teloseq_LC2025/analysis

EPI2ME workflow used to generate analysis outputs:

• wf-teloseq (v1.0.0)

Related Materials

• Multiplex Telo-seq protocol