Oxford Nanopore Open Data project

May 15, 2025
1 min read
Oxford Nanopore Open Data project

The Oxford Nanopore Technologies Open Data project (ont-open-data) provides reference sequencing data from Oxford Nanopore sequencing devices. Data access is provided through the Registry of Open Data on AWS.

The Oxford Nanopore Technologies Open Data project aims to provide exemplar datasets from state of the art of Oxford Nanopore sequencing. Datasets are provided without restriction on availability or use to aid researchers, primarily in the field of genomics and transcriptomics. Our previous data releases have been used to aid the development of new algorithms for small variant and structural variant calling, and in the creation of new single-cell transcriptomics analyses. Data has also been used together with datasets from other sources for the complete telomere to telomere assembly of the Genome In A Bottle sample HG002.

Latest datasets and analyses

The list below represents the most recent dataset or analysis of its class. As new datasets, basecallers, and analysis methods are developed this list will be updated.

Human genomics

DatasetFlow CellKitBasecall modelEPI2ME workflowsDate
Genome in a Bottle Data Release 2025.01FLO-PRO114SQK-LSK114v5.0.0 SUP and HACwf-basecalling, wf-human-variation2025-01-26
Modified Base Best Practices and BenchmarkingFLO-PRO114SQK-LSK114v5.0.0 SUP and HAC2024-10-22
Nanopore-only T2T assembly of a human genomeFLO-PRO114SQK-LSK114v4.3.0 SUP and HAC2024-05-22
Telomere sequencingFLO-PRO114MSQK-LSK114v5.0.0 SUP and HACwf-teloseq2024-05-21
Updated Tumor Normal Pair Benchmark DatasetFLO-PRO114SQK-LSK114v4.2.0 SUP and HACwf-basecalling, wf-somatic-variation2024-03-07
An experimental extremely high-accuracy, ultra-long sequencing kitFLO-PRO114SQK-ULK114Bespoke dorado model2023-12-06
Reduced Representation Methylation Sequencing (RRMS)FLO-MIN106SQK-LSK110v3.32022-07-27
CliveOME cfDNA datasetFLO-PRO114SQK-LSK114v3.5.12022-05-18

Single-cell, transcriptomics and direct RNA

DatasetFlow CellKitBasecall modelEPI2ME workflowsDate
293T and jurkat 10x single cell transcriptomicsFLO-PRO114MSQK-LSK114v5.0.0 SUP and HACwf-single-cell2025-02-17

Synthetic Biology

DatasetFlow CellKitBasecall modelEPI2ME workflowsDate
Plasmid ValidationFLO-MIN114SQK-RBK114v5.0.0 SUP and HACwf-clone-validation2025-04-28

Plant and Animal

DatasetFlow CellKitBasecall modelEPI2ME workflowsDate
Community contributions to Oxford Nanopore Open Data projectFLO-MIN114SQK-LSK114RAW data only2023-01-23

Metagenomics

DatasetFlow CellKitBasecall modelEPI2ME workflowsDate
Zymo Fecal MetagenomeFLO-PRO114MSQK-LSK114v5.0.0 SUP and HACwf-metagenomics2025-05-06

We strive to keep all analyses current, though some may fall behind the more current sequencing runs and basecalling results.

Use of Oxford Nanopore Open Data

The datasets are freely available for download and could be used for:

  1. Exploration of the characteristics of Oxford Nanopore sequence data
  2. Assessment and reproduction of performance benchmarks
  3. Example datasets for testing data analysis with EPI2ME
  4. Development of tools and methods tailored to Oxford Nanopore data

The data deposited showcases sequences from a representative subset of sequencing chemistries. The datasets correspond to publicly-available reference samples including the widely available Genome In A Bottle human reference samples. Raw data are provided with metadata and scripts to describe sample and data provenance.

Archived datasets

Historical datasets can also be found in the repository. Our previous data release blog posts are archived under the data-releases category.

Data access

All data is available from the Registry of Open Data on AWS. See the Oxford Nanopore Open Data Tutorials page for more information.


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Table Of Contents

1
Latest datasets and analyses
2
Human genomics
3
Single-cell, transcriptomics and direct RNA
4
Synthetic Biology
5
Plant and Animal
6
Metagenomics
7
Use of Oxford Nanopore Open Data
8
Archived datasets
9
Data access

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